Association study of rs1333040 and rs1004638 polymorphisms in the 9p21 locus with coronary artery disease in southwest of Iran

Background: Coronary artery disease [CAD] is a multifactorial and heterogenic disease. Recently, genome-wide association studies have reported that rs1333040 [C/T] and rs1004638 [A/T] single nucleotide polymorphisms [SNPs] in the 9p21 locus have very strong association with CAD. This study aimed to examine these associations in Southwest of Iran

Methods: Blood samples were collected from 200 CAD patients and 110 healthy individuals with no CAD. The association of two SNPs with CAD was evaluated by PCR and restriction fragment length polymorphism

Results: Chi-square test showed no association between rs1333040 SNP and CAD [X[2]: 4.66, df: 2, P=0.09]. Also, there was no association between rs1004638 SNP and CAD [X[2]: 0.27, df: 2, P=0.88]

Conclusion: No association was observed between rs1333040 and rs1004638 SNPs in the 9P21 region and CAD in Southwest of Iran

SULF 1 gene polymorphism, rs6990375 is in significant association with fetus failure in IVF technique

Sulfatase 1 [SULF1] function is to remove the 6-O-sulphate group from heparan sulfate. This action changes the binding sites of extracellular growth factors. SULF1 expression has been reported to be changed in angiogenesis. We hypothesized that single nucleotide polymorphisms [SNPs] of SULF1 would impact clinicopathologic characteristics. Study of SULF1 gene polymorphism with fetus failure in in vitro fertilization [IVF] technique. We studied one common [minor allele frequency >0.05] regulatory SNP, rs6990375, with polymerase chain reaction and restriction fragment length polymorphism method, in 53 infertile women with fetus failure in IVF technique and 53 women with at least one healthy child as controls. We found that rs6990375 is significantly associated with an early failure in IVF and frequency of G allele is high in women with fetus failure in IVF technique [p<0.001]. These findings suggest that SULF1genetic variations may play a role in IVF technique fetus failure. Further studies with large sample sizes on SULF1 SNPs may be useful in support of this claim

Relationship between progesterone level on the day of human chorionic gonadotropin administration with outcomes of intra-cytoplasmic sperm injection in infertile couples

Gonadotropin-releasmg hormone agonists or antagonists are used in assisted reproductive technique cycles as premature luteinizing hormone secretion inhibition. Studies have been reported different and contradictory results on the serum progesterone effect on intra-cytoplasmic sperm injection. The purposeof this study was to evaluate the effect of serum progesterone level on the day of Human chorionic gonadotropin [HCG] administration on the intra-cytoplasmic sperm injection [ICSI] outcome in infertile women. 249 infertile couples candidated for ICSI were enrolled in the study. Their serum progesterone level on the day of HCG administration was measured and according to serum level, patients were divided into four groups of less than 0.9, 0.9-1.4, 1.5-1.9, and 7/= ng/mL. The four groups were compared with each other regarding fertility outcomes. Pregnancy rate was not significantly different among the four groups [p>0,05]. Also, there was no significant difference among the groups regarding frequency of abortion and ectopic pregnancy. Serum progesterone level on the day of HCG administration does not have any significant effect on pregnancy outcomes, including abortion, ectopicpregnancy, and pregnancy rate in patients undergoing ICSI treatment